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re: #7 Based on my clinical experience, I think this is way more about the types of people who are likely to receive those sorts of equivocal diagnoses. I'm willing to bet that those patients have some sort of personality pathology mixed with anxious/depressive symptoms (i.e. borderlines/Cluster B/cPTSD/whatever you want to call them) and there is much disagreement between docs about whether or not we should put a diagnostic label on them. It is also very unclear what to do to help them, as medications rarely work, so a diagnostic label is unlikely to help. These patients often engage in self-harm of varying levels of risk (which ends up with them psychiatrically hospitalized, or dead), and almost uniformly have pretty bad interpersonal situations, so (assuming my hypothesis is correct) I'm not surprised to hear that they have poor outcomes.

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Super interesting, thanks for your thoughts!

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From the fly paper: "Freshly dissected Drosophila brains were fixed with 4% PFA for 30 min on ice, before undergoing 0.2% GA fixation on ice under vacuum for another 20 min."

That is impressive! I wonder if the implications for human brain perfusion preservation will hold up...

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